p bad Search Results


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Santa Cruz Biotechnology anti p bad
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GenScript corporation plasmids pcm66tpbad:pct540-phac1437 and pcm66tpbad:hada-phac1437
Plasmids Pcm66tpbad:Pct540 Phac1437 And Pcm66tpbad:Hada Phac1437, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GenScript corporation p-bad antibody
Methylthioadenosine (MTA) promotes cytostatic effects rather than pro-apoptotic effects in melanoma tumors and cells . (A) Tumor sections from mice treated either with DMSO or MTA were stained with cyclin D1 and <t>Ki67</t> antibodies. Representative pictures are shown. (B) Quantification of apoptotic cells within the tumors. Paraffin-embedded tumor samples were subjected to TUNEL assay or stained against cleaved caspase-3. Graph shows the quantification of the TUNEL assay. Positive cells from ten fields (20×) per sample were quantified and the average number of cells per field was calculated. (C) Upper graph, quantification by qRT-PCR of VEGF levels in tumor samples. Lower graph, qRT-PCR of VEGF expression levels. 37-31E were untreated (Control) or treated for 48 h with 10 μM of MTA in complete medium. Microvessel's density quantification in xenografts. Graph shows CD31 fluorescence per μm 2 . Representative pictures are showed on the right. p -values were calculated performing a t -student test. (D) 37-31E cells were treated with MTA (10 μM) for the time points indicated. Fifty micrograms of total lysates were resolved by PAGE-SDS. p-Bad, cleaved-caspase3, p-S6 and cyclin D1 protein levels are showed. GAPDH is used as a loading control. (E) MTA treatment induces a slowdown cell cycle G1 phase. Cells were grown in complete medium for 48 h in the presence or absence of MTA (10 μM). Cell cycle analysis was measured in triplicates using Cell Cycle Analysis Guava-Viacount reagent (Guava Technologies). Average of the three samples in each phase of the cell cycle are shown. p -values were calculated performing a t -student test (* = p < 0.05; ** = p < 0.01).
P Bad Antibody, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ImmunoWay Biotechnology Company rabbit anti-p-bad
Methylthioadenosine (MTA) promotes cytostatic effects rather than pro-apoptotic effects in melanoma tumors and cells . (A) Tumor sections from mice treated either with DMSO or MTA were stained with cyclin D1 and <t>Ki67</t> antibodies. Representative pictures are shown. (B) Quantification of apoptotic cells within the tumors. Paraffin-embedded tumor samples were subjected to TUNEL assay or stained against cleaved caspase-3. Graph shows the quantification of the TUNEL assay. Positive cells from ten fields (20×) per sample were quantified and the average number of cells per field was calculated. (C) Upper graph, quantification by qRT-PCR of VEGF levels in tumor samples. Lower graph, qRT-PCR of VEGF expression levels. 37-31E were untreated (Control) or treated for 48 h with 10 μM of MTA in complete medium. Microvessel's density quantification in xenografts. Graph shows CD31 fluorescence per μm 2 . Representative pictures are showed on the right. p -values were calculated performing a t -student test. (D) 37-31E cells were treated with MTA (10 μM) for the time points indicated. Fifty micrograms of total lysates were resolved by PAGE-SDS. p-Bad, cleaved-caspase3, p-S6 and cyclin D1 protein levels are showed. GAPDH is used as a loading control. (E) MTA treatment induces a slowdown cell cycle G1 phase. Cells were grown in complete medium for 48 h in the presence or absence of MTA (10 μM). Cell cycle analysis was measured in triplicates using Cell Cycle Analysis Guava-Viacount reagent (Guava Technologies). Average of the three samples in each phase of the cell cycle are shown. p -values were calculated performing a t -student test (* = p < 0.05; ** = p < 0.01).
Rabbit Anti P Bad, supplied by ImmunoWay Biotechnology Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Novus Biologicals pbad
Methylthioadenosine (MTA) promotes cytostatic effects rather than pro-apoptotic effects in melanoma tumors and cells . (A) Tumor sections from mice treated either with DMSO or MTA were stained with cyclin D1 and <t>Ki67</t> antibodies. Representative pictures are shown. (B) Quantification of apoptotic cells within the tumors. Paraffin-embedded tumor samples were subjected to TUNEL assay or stained against cleaved caspase-3. Graph shows the quantification of the TUNEL assay. Positive cells from ten fields (20×) per sample were quantified and the average number of cells per field was calculated. (C) Upper graph, quantification by qRT-PCR of VEGF levels in tumor samples. Lower graph, qRT-PCR of VEGF expression levels. 37-31E were untreated (Control) or treated for 48 h with 10 μM of MTA in complete medium. Microvessel's density quantification in xenografts. Graph shows CD31 fluorescence per μm 2 . Representative pictures are showed on the right. p -values were calculated performing a t -student test. (D) 37-31E cells were treated with MTA (10 μM) for the time points indicated. Fifty micrograms of total lysates were resolved by PAGE-SDS. p-Bad, cleaved-caspase3, p-S6 and cyclin D1 protein levels are showed. GAPDH is used as a loading control. (E) MTA treatment induces a slowdown cell cycle G1 phase. Cells were grown in complete medium for 48 h in the presence or absence of MTA (10 μM). Cell cycle analysis was measured in triplicates using Cell Cycle Analysis Guava-Viacount reagent (Guava Technologies). Average of the three samples in each phase of the cell cycle are shown. p -values were calculated performing a t -student test (* = p < 0.05; ** = p < 0.01).
Pbad, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ABclonal Biotechnology p-bad
Methylthioadenosine (MTA) promotes cytostatic effects rather than pro-apoptotic effects in melanoma tumors and cells . (A) Tumor sections from mice treated either with DMSO or MTA were stained with cyclin D1 and <t>Ki67</t> antibodies. Representative pictures are shown. (B) Quantification of apoptotic cells within the tumors. Paraffin-embedded tumor samples were subjected to TUNEL assay or stained against cleaved caspase-3. Graph shows the quantification of the TUNEL assay. Positive cells from ten fields (20×) per sample were quantified and the average number of cells per field was calculated. (C) Upper graph, quantification by qRT-PCR of VEGF levels in tumor samples. Lower graph, qRT-PCR of VEGF expression levels. 37-31E were untreated (Control) or treated for 48 h with 10 μM of MTA in complete medium. Microvessel's density quantification in xenografts. Graph shows CD31 fluorescence per μm 2 . Representative pictures are showed on the right. p -values were calculated performing a t -student test. (D) 37-31E cells were treated with MTA (10 μM) for the time points indicated. Fifty micrograms of total lysates were resolved by PAGE-SDS. p-Bad, cleaved-caspase3, p-S6 and cyclin D1 protein levels are showed. GAPDH is used as a loading control. (E) MTA treatment induces a slowdown cell cycle G1 phase. Cells were grown in complete medium for 48 h in the presence or absence of MTA (10 μM). Cell cycle analysis was measured in triplicates using Cell Cycle Analysis Guava-Viacount reagent (Guava Technologies). Average of the three samples in each phase of the cell cycle are shown. p -values were calculated performing a t -student test (* = p < 0.05; ** = p < 0.01).
P Bad, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Chemiewerk Bad Kostritz GmbH asi-5-p (phosphaden)
Methylthioadenosine (MTA) promotes cytostatic effects rather than pro-apoptotic effects in melanoma tumors and cells . (A) Tumor sections from mice treated either with DMSO or MTA were stained with cyclin D1 and <t>Ki67</t> antibodies. Representative pictures are shown. (B) Quantification of apoptotic cells within the tumors. Paraffin-embedded tumor samples were subjected to TUNEL assay or stained against cleaved caspase-3. Graph shows the quantification of the TUNEL assay. Positive cells from ten fields (20×) per sample were quantified and the average number of cells per field was calculated. (C) Upper graph, quantification by qRT-PCR of VEGF levels in tumor samples. Lower graph, qRT-PCR of VEGF expression levels. 37-31E were untreated (Control) or treated for 48 h with 10 μM of MTA in complete medium. Microvessel's density quantification in xenografts. Graph shows CD31 fluorescence per μm 2 . Representative pictures are showed on the right. p -values were calculated performing a t -student test. (D) 37-31E cells were treated with MTA (10 μM) for the time points indicated. Fifty micrograms of total lysates were resolved by PAGE-SDS. p-Bad, cleaved-caspase3, p-S6 and cyclin D1 protein levels are showed. GAPDH is used as a loading control. (E) MTA treatment induces a slowdown cell cycle G1 phase. Cells were grown in complete medium for 48 h in the presence or absence of MTA (10 μM). Cell cycle analysis was measured in triplicates using Cell Cycle Analysis Guava-Viacount reagent (Guava Technologies). Average of the three samples in each phase of the cell cycle are shown. p -values were calculated performing a t -student test (* = p < 0.05; ** = p < 0.01).
Asi 5 P (Phosphaden), supplied by Chemiewerk Bad Kostritz GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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SuperArray Bioscience Corporation p-bad elisa phosphorylation bad
Methylthioadenosine (MTA) promotes cytostatic effects rather than pro-apoptotic effects in melanoma tumors and cells . (A) Tumor sections from mice treated either with DMSO or MTA were stained with cyclin D1 and <t>Ki67</t> antibodies. Representative pictures are shown. (B) Quantification of apoptotic cells within the tumors. Paraffin-embedded tumor samples were subjected to TUNEL assay or stained against cleaved caspase-3. Graph shows the quantification of the TUNEL assay. Positive cells from ten fields (20×) per sample were quantified and the average number of cells per field was calculated. (C) Upper graph, quantification by qRT-PCR of VEGF levels in tumor samples. Lower graph, qRT-PCR of VEGF expression levels. 37-31E were untreated (Control) or treated for 48 h with 10 μM of MTA in complete medium. Microvessel's density quantification in xenografts. Graph shows CD31 fluorescence per μm 2 . Representative pictures are showed on the right. p -values were calculated performing a t -student test. (D) 37-31E cells were treated with MTA (10 μM) for the time points indicated. Fifty micrograms of total lysates were resolved by PAGE-SDS. p-Bad, cleaved-caspase3, p-S6 and cyclin D1 protein levels are showed. GAPDH is used as a loading control. (E) MTA treatment induces a slowdown cell cycle G1 phase. Cells were grown in complete medium for 48 h in the presence or absence of MTA (10 μM). Cell cycle analysis was measured in triplicates using Cell Cycle Analysis Guava-Viacount reagent (Guava Technologies). Average of the three samples in each phase of the cell cycle are shown. p -values were calculated performing a t -student test (* = p < 0.05; ** = p < 0.01).
P Bad Elisa Phosphorylation Bad, supplied by SuperArray Bioscience Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Verlag GmbH p. jecker klinik für hals-nasen-ohren-heilkunde, klinikum bad salzungen
Methylthioadenosine (MTA) promotes cytostatic effects rather than pro-apoptotic effects in melanoma tumors and cells . (A) Tumor sections from mice treated either with DMSO or MTA were stained with cyclin D1 and <t>Ki67</t> antibodies. Representative pictures are shown. (B) Quantification of apoptotic cells within the tumors. Paraffin-embedded tumor samples were subjected to TUNEL assay or stained against cleaved caspase-3. Graph shows the quantification of the TUNEL assay. Positive cells from ten fields (20×) per sample were quantified and the average number of cells per field was calculated. (C) Upper graph, quantification by qRT-PCR of VEGF levels in tumor samples. Lower graph, qRT-PCR of VEGF expression levels. 37-31E were untreated (Control) or treated for 48 h with 10 μM of MTA in complete medium. Microvessel's density quantification in xenografts. Graph shows CD31 fluorescence per μm 2 . Representative pictures are showed on the right. p -values were calculated performing a t -student test. (D) 37-31E cells were treated with MTA (10 μM) for the time points indicated. Fifty micrograms of total lysates were resolved by PAGE-SDS. p-Bad, cleaved-caspase3, p-S6 and cyclin D1 protein levels are showed. GAPDH is used as a loading control. (E) MTA treatment induces a slowdown cell cycle G1 phase. Cells were grown in complete medium for 48 h in the presence or absence of MTA (10 μM). Cell cycle analysis was measured in triplicates using Cell Cycle Analysis Guava-Viacount reagent (Guava Technologies). Average of the three samples in each phase of the cell cycle are shown. p -values were calculated performing a t -student test (* = p < 0.05; ** = p < 0.01).
P. Jecker Klinik Für Hals Nasen Ohren Heilkunde, Klinikum Bad Salzungen, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Chemiewerk Bad Kostritz GmbH zeolite na-y powder koe-strolith p-tr
Methylthioadenosine (MTA) promotes cytostatic effects rather than pro-apoptotic effects in melanoma tumors and cells . (A) Tumor sections from mice treated either with DMSO or MTA were stained with cyclin D1 and <t>Ki67</t> antibodies. Representative pictures are shown. (B) Quantification of apoptotic cells within the tumors. Paraffin-embedded tumor samples were subjected to TUNEL assay or stained against cleaved caspase-3. Graph shows the quantification of the TUNEL assay. Positive cells from ten fields (20×) per sample were quantified and the average number of cells per field was calculated. (C) Upper graph, quantification by qRT-PCR of VEGF levels in tumor samples. Lower graph, qRT-PCR of VEGF expression levels. 37-31E were untreated (Control) or treated for 48 h with 10 μM of MTA in complete medium. Microvessel's density quantification in xenografts. Graph shows CD31 fluorescence per μm 2 . Representative pictures are showed on the right. p -values were calculated performing a t -student test. (D) 37-31E cells were treated with MTA (10 μM) for the time points indicated. Fifty micrograms of total lysates were resolved by PAGE-SDS. p-Bad, cleaved-caspase3, p-S6 and cyclin D1 protein levels are showed. GAPDH is used as a loading control. (E) MTA treatment induces a slowdown cell cycle G1 phase. Cells were grown in complete medium for 48 h in the presence or absence of MTA (10 μM). Cell cycle analysis was measured in triplicates using Cell Cycle Analysis Guava-Viacount reagent (Guava Technologies). Average of the three samples in each phase of the cell cycle are shown. p -values were calculated performing a t -student test (* = p < 0.05; ** = p < 0.01).
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Image Search Results


Methylthioadenosine (MTA) promotes cytostatic effects rather than pro-apoptotic effects in melanoma tumors and cells . (A) Tumor sections from mice treated either with DMSO or MTA were stained with cyclin D1 and Ki67 antibodies. Representative pictures are shown. (B) Quantification of apoptotic cells within the tumors. Paraffin-embedded tumor samples were subjected to TUNEL assay or stained against cleaved caspase-3. Graph shows the quantification of the TUNEL assay. Positive cells from ten fields (20×) per sample were quantified and the average number of cells per field was calculated. (C) Upper graph, quantification by qRT-PCR of VEGF levels in tumor samples. Lower graph, qRT-PCR of VEGF expression levels. 37-31E were untreated (Control) or treated for 48 h with 10 μM of MTA in complete medium. Microvessel's density quantification in xenografts. Graph shows CD31 fluorescence per μm 2 . Representative pictures are showed on the right. p -values were calculated performing a t -student test. (D) 37-31E cells were treated with MTA (10 μM) for the time points indicated. Fifty micrograms of total lysates were resolved by PAGE-SDS. p-Bad, cleaved-caspase3, p-S6 and cyclin D1 protein levels are showed. GAPDH is used as a loading control. (E) MTA treatment induces a slowdown cell cycle G1 phase. Cells were grown in complete medium for 48 h in the presence or absence of MTA (10 μM). Cell cycle analysis was measured in triplicates using Cell Cycle Analysis Guava-Viacount reagent (Guava Technologies). Average of the three samples in each phase of the cell cycle are shown. p -values were calculated performing a t -student test (* = p < 0.05; ** = p < 0.01).

Journal: BMC Cancer

Article Title: Methylthioadenosine (MTA) inhibits melanoma cell proliferation and in vivo tumor growth

doi: 10.1186/1471-2407-10-265

Figure Lengend Snippet: Methylthioadenosine (MTA) promotes cytostatic effects rather than pro-apoptotic effects in melanoma tumors and cells . (A) Tumor sections from mice treated either with DMSO or MTA were stained with cyclin D1 and Ki67 antibodies. Representative pictures are shown. (B) Quantification of apoptotic cells within the tumors. Paraffin-embedded tumor samples were subjected to TUNEL assay or stained against cleaved caspase-3. Graph shows the quantification of the TUNEL assay. Positive cells from ten fields (20×) per sample were quantified and the average number of cells per field was calculated. (C) Upper graph, quantification by qRT-PCR of VEGF levels in tumor samples. Lower graph, qRT-PCR of VEGF expression levels. 37-31E were untreated (Control) or treated for 48 h with 10 μM of MTA in complete medium. Microvessel's density quantification in xenografts. Graph shows CD31 fluorescence per μm 2 . Representative pictures are showed on the right. p -values were calculated performing a t -student test. (D) 37-31E cells were treated with MTA (10 μM) for the time points indicated. Fifty micrograms of total lysates were resolved by PAGE-SDS. p-Bad, cleaved-caspase3, p-S6 and cyclin D1 protein levels are showed. GAPDH is used as a loading control. (E) MTA treatment induces a slowdown cell cycle G1 phase. Cells were grown in complete medium for 48 h in the presence or absence of MTA (10 μM). Cell cycle analysis was measured in triplicates using Cell Cycle Analysis Guava-Viacount reagent (Guava Technologies). Average of the three samples in each phase of the cell cycle are shown. p -values were calculated performing a t -student test (* = p < 0.05; ** = p < 0.01).

Article Snippet: Cyclin D1 antibody was from Santa Cruz; phospho-Erk1/2 (Thr202/Tyr204), cleaved-caspase-3, p-S6 (Ser235/236), and phospho-Akt (Thr308) antibodies were from Cell Signaling (Danvers, MA USA); p-Bad was from Genscript, Piscataway, NJ, USA, Ki67 was from Master diagnostica, (Granada, Spain) and GAPDH was from Trevigen (Gaitherburg, MD, USA).

Techniques: Staining, TUNEL Assay, Quantitative RT-PCR, Expressing, Control, Fluorescence, Cell Cycle Assay